| Seiyuu | Role |
| Hiroki Touchi | Abel Nightroad |
| Mamiko Noto | Estelle Blanchet |
| Kazuya Nakai | Tres X |
| Takako Honda | Catherina Sforza |
| Toru Ohkawa | William Walter Wordsworth |
| Hitomi Nabatame | Kate Scott |
| Yuki Matsuoka | Seth Nightroad (Episode 11, 15 - 20, 24) |
| Keiji Fujiwara | Isaac Fernando von Kämpfer (Episode 8, 10, 23, 24) |
| Kenichi Suzumura | Dietrich von Lohengrün |
| Juurouta Kosugi | Francesco di Medici |
| Junichi Suwabe | Kain Nightroad (Episode 2, 10, 22 - 24) |
| Fumihiko Tachiki | Vaclav Havel (Episode 5, 9, 10, 20) |
| Junko Minagawa | Ion Fortuna (Episode 12 - 19, 23, 24) |
| Katsuyuki Konishi | Radu Balfon (Episode 12 - 14, 17, 18) |
| Tetsu Inada | Brother Petero (Episode 9, 12 - 14, 20 - 24) |
| Yuko Kaida | Sister Paula (Episode 12 - 14, 20, 22 - 24) |
| Michiko Neya | Astaroshe Aslan (Episode 15 - 19, 23, 24) |
| Yoshinori Fujita | Alessandro XVIII (Episode 1, 4, 5, 9, 10, 20 - 24) |
| Yasunori Matsumoto | Virgil Walsh (Episode 20 - 24) |
| Akiko Kimura | Vanessa Walsh (Episode 20 - 24) |
| Miho Yamada | Mary Spencer (Episode 20 - 24) |
| Tomoko Kawakami | Ellis Wasmeier (Episode 2) |
| Mami Kosuge | Mireille Manson (Episode 2) |
| Kenji Nomura | Marquis of Hungaria (Episode 3 & 4) |
| Atsuko Yuya | Bishop Laura (Episode 3) |
| Sayaka Ohara | Noelle (Episode 5 & 8) |
| Ayako Kawasumi | Young Catherina (Episode 5) |
| Houko Kuwashima | Sister Agnes (Episode 6) |
| Shouto Kashii | Count of Amsterdam (Episode 6) |
| Daisuke Ono | Count of Bruges (Episode 6) |
| Takaya Kuroda | Bidel (Episode 6) |
| Rie Kugimiya | Peter (Episode 7 & 21) |
| Yukari Tamura | Windy (Episode 7 & 21) |
| Fuyuka Ooura | Carley (Episode 7) |
| Susumu Chiba | Count of Zagreb (Episode 11) |
| Yuri Amano | Milka Fortuna (Episode 15, 19, 20) |
| Toshihiko Nakajima | Mimahr (Episode 15 & 16) |
| Masashi Hirose | Chandarl (Episode 15 & 17) |
| Takuma Takewaka | Suleyman (Episode 16 - 19) |
| Ryouko Ono | Jessica (Episode 1) |
| Naoya Uchida | Count of Khartoum (Episode 15, 18, 19) |
| Mai Nakahara | Angelica (Episode 21) |
| Junji Majima | Tom (Episode 21) |
| Satomi Arai | Ginny (Episode 21) |
| Misa Kobayashi | Lilith (Episode 24) |
| Satoshi Hino | Young Abel (Episode 24) |
| Hirofumi Nojima | Young Kain (Episode 24) |